Sample preparation for electron microscopy

The GloQube is a compact, easy to use, stand-alone glow discharge system. The primary application of the system is the hydrophilisation (wetting) of carbon-coated TEM support films and grids. Glow discharge treatment with air will make surfaces negatively charged and hydrophilic and allow the spread of aqueous solutions.

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Freeze-drying is a well-established method to prepare specimens for TEM and SEM. Freeze-drying reduces the distortion and shrinkage effects that occur when a wet sample dries by normal evaporation. The method involves careful sublimation of frozen specimens under vacuum.  

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The modern, solid-state 13.56 MHz plasma reactor K1050X is designed for the requirements of research and development and small-scale production. It is suitable for a wide range of plasma etching/ashing and cleaning applications.

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A vibratome uses a vibrating blade and not pressure to slice through tissue. This method causes little mechanical strain which enables the use of untreated (unembedded) samples. The PELCO easiSlicer combines easy handling with precision technology in an unrivaled, sophisticated tissue slicing instrument.

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Critical point dryer K850 combines versatility and ease of operation. Built-in thermo-electric heating and adiabatic cooling allow precise temperature control. The vertical pressure chamber (32 mm in diameter x 47 mm in height) has a side viewing port which allows a clear view of the liquid meniscus.
K850WM is a compact device for drying a complete 6” wafer.  A convenient wafer holder allows quick transfer and avoids pre-drying.

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Quorum’s critical point dryer model E3100 is well-established in many laboratories and has been used successfully for more than 40 years.  Large valves and two large display instruments for pressure and temperature provide an outstanding control of the drying process. Temperature change is controlled through an optional thermostat (water circuit), which ensures satisfactory cycle times.

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Ted Pella is the leading manufacturer of laboratory microwaves for tissue preparation in electron microscopy, histology and pathology. BioWave works with continuously generated microwaves, thus allowing complete control of the temperature rise. Reproducibility is ensured by programming (time, temperature, power) and an effective microwave distribution system.

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The McIlwain TC752 tissue chopper has been specifically developed for the preparation of microscopic sections of small and irregularly shaped samples (biopsy, small organs etc.). The special design of the instrument ensures that the tissue is sectioned with a minimum of functional disturbance, and facilitates the repeatable preparation of tissue sections.

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Flecto-PC-MFC is a compact and cost-effective instrument for laboratory use and small-volume applications. The implemented KHz generator technique eliminates the need for sophisticated electronics and impedance adaptation as is required for MHz generators.

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