GloQube Glow discharge system
from Quorum Technologies
The GloQube is a compact, easy to use, stand-alone glow discharge system. The primary application of the system is the hydrophilisation (wetting) of carbon-coated TEM support films and grids. Glow discharge treatment with air will make surfaces negatively charged and hydrophilic and allow the spread of aqueous solutions.
|Dual or single chamber|
|Hydrophilic/hydrophobic and negative/positive modes|
|Fully automatic, short process times, touch screen control|
|Safe vapour delivery using septum-sealed vials|
|Automatic valving between chambers to prevent cross-contamination|
The GloQube is a compact, easy to use, stand-alone glow discharge system.
The primary application of the GloQube is the hydrophilisation (wetting) of carbon-coated transmission electron microscopy (TEM) support films and grids which otherwise have the tendency to be hydrophobic. Glow discharge treatment with air/oxygen will make film surfaces negatively charged and hydrophilic and allow the easy spread of aqueous solutions. This and other processes are outlined below.
|Surface state||Charge||Atmosphere||Typical applications|
|Hydrophilic||Negative||Air||Carbon coated TEM grids|
|Hydrophilic||Positive||Air with magnesium acetate post-treatment||Nucleic acid adhesion to carbon films|
|Hydrophobic||Positive||Alkylamine||Proteins, antibodies and nucleic acids|
|Hydrophobic||Negative||Air||Positively charged protein molecules, (e.g. ferritin,|
The GloQube® is available in two formats: The GloQube-D has two independent vacuum chambers: a clean chamber for applications requiring hydrophobic/hydrophilic conversion, typically using air as the process gas, and a vapour chamber designed for hydrophilic/hydrophobic (negative or positive) conversions, typically using reagents such as methanol and alkylamine. The GloQube-S has a single clean chamber.
With operator safety and ease of use firmly in mind, reusable septum-sealed reagent vials are used. Loading and removing reagents is both convenient and reliable and ensures minimal user contact with the reagents. The vial is located in its holder and inserted into a shielded needle using a simple twist-and-push home motion.
Each chamber can accommodate two 25 x 75 mm glass microscopes slides. Loading could not be easier using draw-style chamber doors and specimen stages. The stages are height adjustable and fitted with removable glass slide holders. For additional convenience – and to allow easy access for chamber cleaning – the stages can be completely removed.
The GloQube-D has automatic valving between chambers which maintains cleanliness by preventing cross-contamination. At the end of a process run, automatic "soft" venting to atmosphere through filtered inlets ensures that delicate samples, such as TEM grids, are not disturbed.
Both GloQube-D and GloQube-S require a single vacuum pump working in the 0.1 to 1 mbar range. A typical pump time to operational vacuum is 60 seconds. A 750 mm flexible stainless vacuum hose is supplied with the GloQube.
|Plasma current||1-40 mA|
|HV power supply||30 W|
|Maximum voltage||800 V|
|Electrode polarity – clean chamber||DC glow positive DC glow negative|
|Electrode polarity – vapour chamber||DC glow positive DC glow negative|
|Sample stage||125 x 100 mm (4.9” x 3.94”) for two 25 x 75 mm (1” x 3”) slides|
|Sample stage operational heights||Adjustable 12.5 mm (0.5”), 22.5 mm (0.9”) or 35 mm (1.38”)|
|Pump hold time||0-24 hours|
|Process time||1-600 seconds|
|Vacuum control/ working range||Integrated pirani gauge, 0.1 to 1 mbar|
|Pump minimum requirements||6 m3/hr, 3600 l/m, 0.03 mbar ultimate vacuum.|
|chamber vent inlets||Filtered air inlets with slow vent to minimise sample disturbance|
|On-board reagent storage||Reagents are contained in reusable sealed glass vials to minimise exposure to hazards|
|High voltage safety interlocks||Hardware safety interlocked and software for process control|
|Chamber size||100 mm W x 100 mm H x 127 mm D (3.94” x 3.94” x 5”)|
|Instrument size/weight||366 mm W x336 mm H x 364 mm D (13.2” x 14.3”) 19.5 kg (42.9 lbs.) (GloQube-D)|
|Power requirements||120 V 60 Hz, 15 A or 230 V 50 Hz, 10 A|
|Communication port||Ethernet port for instrument software updates|